Overexpression of poplar cellulase accelerates growth and disturbs the closing movements of leaves in sengon.
Identifieur interne : 003852 ( Main/Exploration ); précédent : 003851; suivant : 003853Overexpression of poplar cellulase accelerates growth and disturbs the closing movements of leaves in sengon.
Auteurs : Sri Hartati [Indonésie] ; Enny Sudarmonowati ; Yong Woo Park ; Tomomi Kaku ; Rumi Kaida ; Kei'Ichi Baba ; Takahisa HayashiSource :
- Plant physiology [ 0032-0889 ] ; 2008.
Descripteurs français
- KwdFr :
- Amorces ADN (MeSH), Cellulase (métabolisme), Données de séquences moléculaires (MeSH), Feuilles de plante (croissance et développement), Feuilles de plante (physiologie), Populus (croissance et développement), Populus (enzymologie), Populus (génétique), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), RT-PCR (MeSH), Séquence nucléotidique (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- croissance et développement : Feuilles de plante, Populus.
- enzymologie : Populus.
- génétique : Populus, Protéines recombinantes.
- métabolisme : Cellulase, Protéines recombinantes.
- physiologie : Feuilles de plante.
- Amorces ADN, Données de séquences moléculaires, RT-PCR, Séquence nucléotidique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Base Sequence (MeSH), Cellulase (metabolism), DNA Primers (MeSH), Molecular Sequence Data (MeSH), Plant Leaves (growth & development), Plant Leaves (physiology), Plants, Genetically Modified (MeSH), Populus (enzymology), Populus (genetics), Populus (growth & development), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Reverse Transcriptase Polymerase Chain Reaction (MeSH).
- MESH :
- chemical , genetics : Recombinant Proteins.
- chemical , metabolism : Cellulase, Recombinant Proteins.
- enzymology : Populus.
- genetics : Populus.
- growth & development : Plant Leaves, Populus.
- physiology : Plant Leaves.
- Base Sequence, DNA Primers, Molecular Sequence Data, Plants, Genetically Modified, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
In this study, poplar (Populus alba) cellulase (PaPopCel1) was overexpressed in a tropical Leguminosae tree, sengon (Paraserianthes falcataria), by the Agrobacterium tumefaciens method. PaPopCel1 overexpression increased the length and width of stems with larger leaves, which showed a moderately higher density of green color than leaves of the wild type. The pairs of leaves on the transgenic plants closed more slowly during sunset than those on the wild-type plants. When main veins from each genotype were excised and placed on a paper towel, however, the leaves of the transgenic plants closed more rapidly than those of the wild-type plant. Based on carbohydrate analyses of cell walls, the leaves of the transgenic plants contained less wall-bound xyloglucan than those of the wild-type plants. In situ xyloglucan endotransglucosylase activity showed that the incorporation of whole xyloglucan, potentially for wall tightening, occurred in the parenchyma cells (motor cells) of the petiolule pulvinus attached to the main vein, although the transgenic plant incorporated less whole xyloglucan than the wild-type plant. These observations support the hypothesis that the paracrystalline sites of cellulose microfibrils are attacked by poplar cellulase, which loosens xyloglucan intercalation, resulting in an irreversible wall modification. This process could be the reason why the overexpression of poplar cellulase both promotes plant growth and disturbs the biological clock of the plant by altering the closing movements of the leaves of the plant.
DOI: 10.1104/pp.108.116970
PubMed: 18417637
PubMed Central: PMC2409039
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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uniqKey="Kaku T" first="Tomomi" last="Kaku">Tomomi Kaku</name></author><author><name sortKey="Kaida, Rumi" sort="Kaida, Rumi" uniqKey="Kaida R" first="Rumi" last="Kaida">Rumi Kaida</name></author><author><name sortKey="Baba, Kei Ichi" sort="Baba, Kei Ichi" uniqKey="Baba K" first="Kei'Ichi" last="Baba">Kei'Ichi Baba</name></author><author><name sortKey="Hayashi, Takahisa" sort="Hayashi, Takahisa" uniqKey="Hayashi T" first="Takahisa" last="Hayashi">Takahisa Hayashi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18417637</idno><idno type="pmid">18417637</idno><idno type="doi">10.1104/pp.108.116970</idno><idno type="pmc">PMC2409039</idno><idno type="wicri:Area/Main/Corpus">003920</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003920</idno><idno type="wicri:Area/Main/Curation">003920</idno><idno type="wicri:explorRef" wicri:stream="Main" 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last="Park">Yong Woo Park</name></author><author><name sortKey="Kaku, Tomomi" sort="Kaku, Tomomi" uniqKey="Kaku T" first="Tomomi" last="Kaku">Tomomi Kaku</name></author><author><name sortKey="Kaida, Rumi" sort="Kaida, Rumi" uniqKey="Kaida R" first="Rumi" last="Kaida">Rumi Kaida</name></author><author><name sortKey="Baba, Kei Ichi" sort="Baba, Kei Ichi" uniqKey="Baba K" first="Kei'Ichi" last="Baba">Kei'Ichi Baba</name></author><author><name sortKey="Hayashi, Takahisa" sort="Hayashi, Takahisa" uniqKey="Hayashi T" first="Takahisa" last="Hayashi">Takahisa Hayashi</name></author></analytic><series><title level="j">Plant physiology</title><idno type="ISSN">0032-0889</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence (MeSH)</term><term>Cellulase (metabolism)</term><term>DNA Primers (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Plant Leaves (growth & development)</term><term>Plant Leaves (physiology)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (enzymology)</term><term>Populus (genetics)</term><term>Populus (growth & development)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (MeSH)</term><term>Cellulase (métabolisme)</term><term>Données de séquences moléculaires (MeSH)</term><term>Feuilles de plante (croissance et développement)</term><term>Feuilles de plante (physiologie)</term><term>Populus (croissance et développement)</term><term>Populus (enzymologie)</term><term>Populus (génétique)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>RT-PCR (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulase</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Feuilles de plante</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulase</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Feuilles de plante</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Plant Leaves</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>DNA Primers</term><term>Molecular Sequence Data</term><term>Plants, Genetically Modified</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Données de séquences moléculaires</term><term>RT-PCR</term><term>Séquence nucléotidique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study, poplar (Populus alba) cellulase (PaPopCel1) was overexpressed in a tropical Leguminosae tree, sengon (Paraserianthes falcataria), by the Agrobacterium tumefaciens method. PaPopCel1 overexpression increased the length and width of stems with larger leaves, which showed a moderately higher density of green color than leaves of the wild type. The pairs of leaves on the transgenic plants closed more slowly during sunset than those on the wild-type plants. When main veins from each genotype were excised and placed on a paper towel, however, the leaves of the transgenic plants closed more rapidly than those of the wild-type plant. Based on carbohydrate analyses of cell walls, the leaves of the transgenic plants contained less wall-bound xyloglucan than those of the wild-type plants. In situ xyloglucan endotransglucosylase activity showed that the incorporation of whole xyloglucan, potentially for wall tightening, occurred in the parenchyma cells (motor cells) of the petiolule pulvinus attached to the main vein, although the transgenic plant incorporated less whole xyloglucan than the wild-type plant. These observations support the hypothesis that the paracrystalline sites of cellulose microfibrils are attacked by poplar cellulase, which loosens xyloglucan intercalation, resulting in an irreversible wall modification. This process could be the reason why the overexpression of poplar cellulase both promotes plant growth and disturbs the biological clock of the plant by altering the closing movements of the leaves of the plant.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18417637</PMID><DateCompleted><Year>2008</Year><Month>08</Month><Day>05</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0032-0889</ISSN><JournalIssue CitedMedium="Print"><Volume>147</Volume><Issue>2</Issue><PubDate><Year>2008</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Plant physiology</Title><ISOAbbreviation>Plant Physiol</ISOAbbreviation></Journal><ArticleTitle>Overexpression of poplar cellulase accelerates growth and disturbs the closing movements of leaves in sengon.</ArticleTitle><Pagination><MedlinePgn>552-61</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1104/pp.108.116970</ELocationID><Abstract><AbstractText>In this study, poplar (Populus alba) cellulase (PaPopCel1) was overexpressed in a tropical Leguminosae tree, sengon (Paraserianthes falcataria), by the Agrobacterium tumefaciens method. PaPopCel1 overexpression increased the length and width of stems with larger leaves, which showed a moderately higher density of green color than leaves of the wild type. The pairs of leaves on the transgenic plants closed more slowly during sunset than those on the wild-type plants. When main veins from each genotype were excised and placed on a paper towel, however, the leaves of the transgenic plants closed more rapidly than those of the wild-type plant. Based on carbohydrate analyses of cell walls, the leaves of the transgenic plants contained less wall-bound xyloglucan than those of the wild-type plants. In situ xyloglucan endotransglucosylase activity showed that the incorporation of whole xyloglucan, potentially for wall tightening, occurred in the parenchyma cells (motor cells) of the petiolule pulvinus attached to the main vein, although the transgenic plant incorporated less whole xyloglucan than the wild-type plant. These observations support the hypothesis that the paracrystalline sites of cellulose microfibrils are attacked by poplar cellulase, which loosens xyloglucan intercalation, resulting in an irreversible wall modification. This process could be the reason why the overexpression of poplar cellulase both promotes plant growth and disturbs the biological clock of the plant by altering the closing movements of the leaves of the plant.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hartati</LastName><ForeName>Sri</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Research Centre for Biotechnology, LIPI, Cibinong 16911, Indonesia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sudarmonowati</LastName><ForeName>Enny</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Park</LastName><ForeName>Yong Woo</ForeName><Initials>YW</Initials></Author><Author ValidYN="Y"><LastName>Kaku</LastName><ForeName>Tomomi</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Kaida</LastName><ForeName>Rumi</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Baba</LastName><ForeName>Kei'ichi</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Hayashi</LastName><ForeName>Takahisa</ForeName><Initials>T</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>D32166</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>04</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Physiol</MedlineTA><NlmUniqueID>0401224</NlmUniqueID><ISSNLinking>0032-0889</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA 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Reaction</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>4</Month><Day>18</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>8</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>4</Month><Day>18</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18417637</ArticleId><ArticleId IdType="pii">pp.108.116970</ArticleId><ArticleId IdType="doi">10.1104/pp.108.116970</ArticleId><ArticleId IdType="pmc">PMC2409039</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Plant Cell Physiol. 1995 Jun;36(4):607-14</Citation><ArticleIdList><ArticleId IdType="pubmed">7640890</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2003 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uniqKey="Sudarmonowati E" first="Enny" last="Sudarmonowati">Enny Sudarmonowati</name></noCountry><country name="Indonésie"><noRegion><name sortKey="Hartati, Sri" sort="Hartati, Sri" uniqKey="Hartati S" first="Sri" last="Hartati">Sri Hartati</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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